Refer to CBSE Class 12 Biology Biotechnology and Its Applications MCQs provided below. CBSE Class 12 Biology MCQs with answers available in Pdf for free download. The MCQ Questions for Class 12 Biology with answers have been prepared as per the latest syllabus, CBSE books and examination pattern suggested in Class 12 by CBSE, NCERT and KVS. Multiple Choice Questions for Chapter 12 Biotechnology and its Applications are an important part of exams for Class 12 Biology and if practiced properly can help you to get higher marks. Refer to more Chapter-wise MCQs for CBSE Class 12 Biology and also download more latest study material for all subjects
MCQ for Class 12 Biology Chapter 12 Biotechnology and its Applications
Class 12 Biology students should refer to the following multiple-choice questions with answers for Chapter 12 Biotechnology and its Applications in Class 12. These MCQ questions with answers for Class 12 Biology will come in exams and help you to score good marks
Chapter 12 Biotechnology and its Applications MCQ Questions Class 12 Biology with Answers
Question : Consumption of which one of the following foods can prevent the kind of blindness associated with vitamin ‘A’ deficiency?
a) ‘Flavr Savr’ tomato
b) Canolla
c) Golden rice
d) Bt-Brinjal
Answer : C
Question : Bacillus thuringiensis (Bt) strains have been used for designing novel
a) bio-fertilizers
b) bio-metallurgical techniques
c) bio-mineralization processes
d) bio-insecticidal plants
Answer : D
Question : The genetically-modified (GM) brinjal in India has been developed for
a) insect-resistance
b) enhancing shelf life
c) enhancing mineral content
d) drought-resistance
Answer : A
Question : Bt toxin kill the larvae of certain insects
a) by binding of activated toxin on mid gut epithelial cells, creating pores, leading to swelling and lysis.
b) by stopping transcription of larval cells.
c) by altering central dogma taking place in the cells of gut of larva.
d) by stopping protein synthesis.
Answer : A
Question : Cry protein is obtained from
a) Bacillus thuringiensis
b) Bacillus subtilis
c) Clostridium welchi
d) E. coli
Answer : A
Question : Cry-genes have been introduced in
a) cotton and corn
b) rice
c) potato and soyabean
d) all of the above
Answer : D
Question : Bt toxin is harmful to insects like
a) lepidoterans (tobacco budworm, armyworms)
b) coleopterans (beetles)
c) dipterans (flies and mosquito)
d) all of the above
Answer : D
Question : RNA interference (RNAi) technique has been devised to protect the plants from nematode is silenced by ______produced by the host plant
a) dsDNA
b) ssDNA
c) dsRNA
d) target proteins
Answer : C
Question : Tobacco plants resistant to a nematode have been developed by the introduction of DNA that produced (in the host cells)
a) both sense and anti-sense RNA.
b) a particular hormone.
c) an antifeedant.
d) a toxic protein.
Answer : A
Question : C-peptide of human insulin is
a) a part of mature insulin molecule.
b) responsible for formation of disulphide bridges.
c) removed during maturation of pro-insulin to insulin.
d) responsible for its biological activity.
Answer : C
Question : Bt cotton is not
a) Resistant to all pesticides
b) A bacterial gene expressing system
c) Insect resistant
d) A GM plant
Answer : A
Question : C-peptide of human insulin is
a) Removed during maturation of pro-insulin to insulin
b) Responsible for its biological activity
c) Responsible for formation of disulphide bridges
d) A part of mature insulin molecule
Answer : A
Question : GEAC stands for
a) Genetic Engineering Approval Committee
b) Genetic and Environment Approval committee
c) Ground Environment Action Committee
d) Genome Engineering Action Committee
Answer : A
Question : α - 1 antitrypsin is :
a) Used to treat emphysema
b) Used to treat arthritis
c) An enzyme
d) An antacid
Answer : A
Question : A probe which is a molecule used to locate specific sequences in a mixture of DNA or RNA molecules could be
a) Either RNA or DNA
b) Can be ss DNA but not ss RNA
c) A single stranded DNA
d) A single stranded RNA
Answer : A
Question : ———– is used as a vector for cloning into higher organisms
a) Retrovirus
b) Baculovirus
c) Salmonella typhimurium
d) All of these
Answer : A
Question : Which bacterium is used in the production of insulin by genetic engineering?
a) Escherichia
b) Mycobacterium
c) Rhizobium
d) Saccharomyces
Answer : A
Question : Restriction endonuclease is employed for cutting
a) Double strand DNA
b) RNA fragment
c) mRNA
d) A single strand DNA
Answer : A
Question : Production of human protein in bacteria by genetic engineering is possible because
a) Genetic code is universal
b) Bacterial cell can undertake RNA splicing
c) Mechanism of gene regulation is identical in humn and bacteria
d) None of these
Answer : A
Question : Some of the characteristics of Bt cotton are
a) High yield and production of toxic proteins ceystals which kill dipteran pests
b) High yield and resistance to bollworms
c) High yield and resistance to bollworms
d) All of these
Answer : A
Question : Choose the correct option regarding Retrovirus
a) An RNA virus that can synthesise DNA during infection
b) A DNA virus that can synthesise RNA during infection
c) A ssDNA virus
d) A dsRNA virus
Answer : A
Question : The site of production of ADA in the body is
a) Lymphocytes
b) Bone marrow
c) Blood plasma
d) Monocytes
Answer : A
Question : A protoxin is:
a) Inactive toxin
b) Toxin produced by protozoa
c) A denatured toxin
d) A primitive toxin
Answer : A
Question : Pathophysiology is the
a) Study of altered physiology of host
b) None of the above
c) Study of normal physiology of host
d) Study of physiology of pathogen
Answer : A
Question : The trigger for activation of toxin of Bacillus thuringiensis is
a) Alkaline pH of gut
b) Mechanical action in the insect gut
c) High temperature
d) Acidic pH of stomach
Answer : A
Question : Clot buster obtained from Streptococcus and modified by genetic engineering is
a) Streptokinase
b) Penicillin
c) Cyclosporin A
d) Statins
Answer : A
Question : Maturation of proinsulin into insulin takes place after
a) Maturation of proinsulin into insulin takes place after
b) Joining of c-peptide
c) Addition of disulphide bridge
d) Joining of c-peptide
Answer : A
Question : Restriction enzymes are also called
a) Molecular scissors
b) Carriers
c) Vectors
d) Molecular makers
Answer : A
Question : RNA interference helps in
a) Cell defence
b) Cell differentiation
c) Micropropagation
d) Cell proliferation
Answer : A
Question : Which of the following is the quality of improved transgenic basmati rice?
a) Gives high yield and is rich in vitamin A
b) Does not require chemical fertilizers and growth hormones
c) Resistant to insects and diseases
d) Resistant to insects and diseases
Answer : A
Question : The first human drug made using recombinant DNA technology was
a) glyphosatase
b) TPA
c) insulin
d) erythropoietin
Answer : C
Question : E. coli are used in production of
a) rifampicin
b) LH
c) ecdysone
d) interferon
Answer : D
Question : The first clinical gene therapy was given in 1990 to a 4 years old girl with enzyme deficiency of
a) adenosine deaminase (ADA)
b) tyrosine oxidase
c) monamine oxidase
d) glutamate dehydrogenase
Answer : A
Question : In some children, ADA deficiency can be cured by
a) bone marrow transplantation
b) enzyme replacement therapy
c) both a) and b)
d) none of the above
Answer : C
Question : The site of production of ADA in the body is
a) bone marrow
b) lymphocytes
c) blood plasma
d) monocytes
Answer : B
Question : Genes of interest can be selected from a genomic library by using
a) restriction enzymes
b) cloning vectors
c) DNA probes
d) gene targets
Answer : C
Question : DNA or RNA segment tagged with a radioactive molecule is called ___________ .
a) vector
b) probe
c) clone
d) plasmid
Answer : B
Question : The transgenic animals are those which have
a) foreign DNA in some cells.
b) foreign DNA in all of their cells.
c) foreign RNA in all of their cells.
d) both a) and c)
Answer : B
Question : Today, transgenic models exist for many human diseases which includes
(i) Cancer (ii) Cystic fibrosis
(iii) Rheumatoid arthritis (iv) Alzhiemer’s disease
a) (i) and (iii) only
b) (ii) and (iii) only
c) (i), (ii) and (iii) only
d) all of these
Answer : D
Question : The protein a-1 antitrypsin is used to treat the
a) cancer
b) rheumatoid arthritis
c) Alzheimer’s disease
d) emphysema
Answer : D
Question: Golden rice is
a) A transgenic rice having gene for - carotene
b) Wild variety of rice with yellow coloured grains
c) Long stored rice having yellow colour tint
d) A variety of rice grown along the yellow river in China
Answer : A
Question: In RNAi, genes are silenced using
a) ds RNA
b) ss RNA
c) ds DNA
d) ss DNA
Answer : A
Question: The first clinical gene therapy was done for the treatment of
a) SCID (Servere Combined Immuno Deficiency resulting form deficiency of ADA)
b) Cystic fibrosis
c) Cancer
d) AIDS
Answer : A
Question: ADA is an enzyme which is deficient in a genetic disorder SCID. What is the full form of ADA?
a) Adenosine deaminase
b) Aspartate deaminase
c) Arginine deaminase
d) Adenosine deoxy aminase
Answer : A
Question: Silencing of a gene could be achieved through the use of
a) by both
b) none of the above
c) antisense RNA
d) short interfering RNA (RNAi
Answer : A
Question: In genetics engineering which is used for transfer of genes from one cell to another
a) Vector
b) Probe
c) Virus
d) Plasmid
Answer : A
Question: Recombinant DNA or rDNA technology as discovered by
a) Cohen and Boyer
b) Sutton and Avery
c) Sutton and Avery
d) All of these
Answer : A
Question: What is true of Bt toxin?
a) The inactive protoxin get converted into active form in the insect gut
b) Bt protein exists as active toxin in bacillus
c) The concerned bacillus has antitoxins
d) None of these
Answer : A
Question: Which is a genetically modified crop?
a) All of these
b) Golden rice
c) Bt-brinjal
d) Bt-cotton
Answer : A
Question: PCR technique was invented by
a) Karry Mullis
b) Boyer
c) Sanger
d) None of these
Answer : A
Question : The transgenic animals are those which have
a) foreign DNA in some of its cells
b) foreign DNA in all its cells
c) foreign RNA in all its cells
d) DNA and RNA both in the cells
Answer: B
Question : Genetically engineered bacteria have been successfully used in the commercial produc- tion of
a) human insulin
b) testosterone
c) thyroxine
d) melatonin
Answer: A
Question : The first successfully cloned mammals (ani- mal) that gained worldwide publicity was
a) Molly (a sheep)
b) Polly (a sheep)
c) Chance (a bull)
d) Dolly (a sheep)
Answer: D
Question : Producing a giant mouse in the laboratory was possible through
a) gene mutation
b) gene manipulation
c) gene synthesis
d) gene duplication
Answer: A
Question : Production of a human protein in bacteria by genetic engineering is possible because
a) bacterial cell can carry out the RNA splicing reactions
b) the human chromosome can replicate in bacterial cell
c) the mechanism of gene regulation is identical in humans and bacteria
d) the genetic code is universal
Answer: D
Question : Golden rice is a transgenic crop of the future with the following improved trait
a) high lysine (essential amino acid) content
b) insect resistance
c) high protein content
d) high vitamin-A content
Answer: D
Question : Cry-I endotoxins obtained from Bacillus thuringiensis are effective against
a) mosquitoes
b) flies
c) nematodes
d) bollworms
Answer: B
Question : The bacterium Bacillus thuringiensis is widely used in contemporary biology as
a) Agent for production of dairy products
b) Source of industrial enzyme
c) Indicator of water pollution
d) Insecticide
Answer: D
Question : Transgenic plants are the ones
a) produced after protoplast fusion in arti- ficial medium.
b) grown in artificial medium after hybrid- ization in the field.
c) produced by a somatic embryo in artifi- cial medium.
d) generated by introducing foreign DNA in to a cell and regenerating a plant from that cell.
Answer: D
Question : The genetic defect - adenosine deaminase (ADA) deficiency may be cured permanently by
a) introducing bone marrow cells produc- ing ADA into cells at early embryonic stages.
b) enzyme replacement therapy.
c) periodic infusion of genetically engi- neered lymphocytes having functional ADA cDNA.
d) administering adenosine deaminase activators.
Answer: C
Question : What is true about Bt toxin?
a) The activated toxin enters the ovaries of the pest to sterilise it and thus prevent its multiplication.
b) The concerned Bacillus has antitoxins.
c) The inactive protoxin gets converted into active form in the insect gut.
d) Bt protein exists as active toxin in the Bacillus.
Answer: C
Question : The genetically-modified (GM) brinjal in India has been developed for
a) Insect-resistance
b) Enhancing self life
c) Enhancing mineral content
d) Drought-resistance
Answer: A
Question : Genetic engineering has been successfully used for producing
a) transgenic mice for testing safety of polio vaccine before use in humans
b) transgenic models for studying new treatments for certain cardiac diseases
c) transgenic Cow-Rosie which produces high fat milk for making ghee
d) animals like bulls for farm work as they have super power
Answer: B
Question : Some of the characteristics of Bt cotton are
a) Long fibre and resistance to aphids
b) Medium yield, long fibre and resistance to beetle pests
c) high yield and production of toxic protein crystals which kill dipteran pests
d) High yield and resistance to bollworms
Answer: D
Question : An improved variety of transgenic basmati rice
a) does not require chemical fertilizers and growth hormones
b) gives high yield and is rich in vitamin A
c) is completely resistant to all insect pests and diseases of paddy
d) gives high yield but has no characteristic aroma
Answer: C
Question : Read the following four statements (A-D) about certain mistakes in two of them.
(A) The first transgenic buffalo, Rosie pro- duced milk which was human alpha-lac- talbumin enriched.
(B) Restriction enzymes are used in isolation of DNA from other macro molecules.
(C) Downstream processing is one of the steps of R-DNA technology.
(D) Disarmed pathogen vectors are also used in transfer of R-DNA into the host.
Which are the two statements having mis- takes ?
a) Statements (A) and (B)
b) Statements (B) and (C)
c) Statements (C) and (D)
d) Statements (A) and (C)
Answer: A
Question : Maximum number of existing transgenic animals is of
a) Fish
b) Mice
c) Cow
d) Pig
Answer: B
Question : The process of RNA interference has been used in the development of plants resistant to
a) Nematodes
b) Fungi
c) Viruses
d) Insects
Answer: A
Question : Which one is a true statement regarding DNA polymerase used in PCR ?
a) It is isolated from a virus
b) It remains active at high temperature
c) It is used to ligate introduced DNA in recipient cells
d) It serves as a selectable marker
Answer: B
Question : Consumption of which one of the following foods can prevent the kind of blindness as- sociated with vitamin ‘A’ deficiency
a) Golden rice
b) Bt-Brinjal
c) Flaver Savr tomato
d) Canolla
Answer: A
Question : The first human hormone produced by re- combinant DNA technology is -
a) Insulin
b) Estrogen
c) Thyroxine
d) Progesterone
Answer: A
Question : The crops engineered for glyphosate are re- sistant/ tolerant to :-
a) Bacteria
b) Insects
c) Herbicides
d) Fungi
Answer: C
Question : In Bt cotton, the Bt toxin present in plant tis- sue as pro-toxin is converted into active toxin due to :-
a) Acidic pH of the insect gut
b) Action of gut micro-organisms
c) Presence of conversion factors in insect gut
d) Alkaline pH of the insect gut
Answer: D
Question : Which body of the Government of India regu- lates GM research and safety of introducing GM organisms for public services ?
a) Indian Council of Agricultural Research
b) Genetic Engineering Approval Committee
c) Research Committeeon Genetic Manipulation
d) Bio-safety committee
Answer: B
Question : Golden rice is a genetically modified crop plant where the incorporated gene is meant for biosynthesis of :
a) Vitamin A
b) Vitamin B
c) Vitamin C
d) Omega 3
Answer: A
Case-based MCQs
I. Read the following text and answer the following questions on the basis of the same :
Transgenic animals have their DNA manipulated to possess and express an extra gene. The genome of these animals has been changed and they can carry genes from other species. The first transgenic sheep was Tracy. The reason behind producing these transgenic animals are for specific economic trait, some are produced as disease models (animals genetically manipulated to exhibit disease symptoms so that effective treatment can be studied).
Question : ___ were the first mammals to be transgenic animals.
(A) Sheep
(B) Mice
(C) Fruit flies
(D) None of these
Answer : B
Question : 95% transgenic animals are :
(A) Mice
(B) Cat
(C) Dog
(D) Sheep
Answer : A
Question : The first transgenic cow, Rosie, was produced in :
(A) 1995
(B) 1996
(C) 1997
(D) 1998.
Answer : C
Question : First cloned animal is :
(A) Rat
(B) Rabbit
(C) Pig
(D) Sheep.
Answer : D
Directions : In the following questions a statement of assertion (A) is followed by a statement of reason
(R). Mark the correct choice as :
(A) Both assertion (A) and reason (R) are true and reason (R) is the correct explanation of assertion (A).
(B) Both assertion (A) and reason (R) are true but reason (R) is not the correct explanation of assertion (A).
(C) Assertion (A) is true but reason (R) is false.
(D) Assertion (A) is false but reason (R) is true.
Question : Assertion (A) : Dolly, was the first mammal to be cloned from an udder cell.
Reason (R) : To create, Dolly the Finn Dorset ewe, Ian Wilmut and Keith Campbell used aid from three mothers.
Answer : A.
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